Loss of WNK1 Suppressed the Malignant Behaviors of Hepatocellular Carcinoma Cells by Promoting Autophagy and Activating AMPK Pathway

Figure 4

Loss of WNK1 restricted the malignant behaviors of HCC cells by suppressing autophagy and inactivating AMPK pathway. (a, b) GSK690693 treatment reduced the expression patterns of p-AMPK, Beclin 1, LC3 II/I, and increased P62 level, and the above results were reversed after si-WNK1 treatment. (c, d) GSK690693 treatment increased the OD values of Huh7 and Hep3B cells, while si-WNK1 treatment counteracted the effect of GSK690693 on the OD values of Huh7 and Hep3B cells. (e–g) GSK690693 treatment increased the clone number of Huh7 and Hep3B cells, while si-WNK1 treatment counteracted the effect of GSK690693 on the clone number of Huh7 and Hep3B cells. , vs. control, ^## vs. si-WNK1, ^&, ^&& vs. GSK690693.

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