Serum Soluble CD89-IgA Complexes Are Elevated in IgA Nephropathy without Immunosuppressant History

Figure 1

Measurement of serum sCD89-IgA complexes. (a) Detection by sandwich ELISA. Microtiter plates were coated with mouse anti-CD89 IgG (MIP8a, MIP15b, MIP38c, or MIP71a, respectively). Recombinant sCD89 was added into sCD89-free serum at indicated concentrations. The bound sCD89-IgA complexes were detected by HRP-conjugated anti-IgA antibody, KT40. (b) Detection of sCD89-IgA complexes by Western blotting. Recombinant sCD89 was added into the sCD89-free serum at indicated concentrations. After incubation at 4°C overnight, sCD89-IgA complexes were pulled down by MIP15b-beads. The sCD89-IgA complexes absorbed on the beads were run on SDS-PAGE, and IgA and CD89 were analyzed separately by Western blotting (see Subjects and Methods).

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