Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma

Figure 7

FEN1 promotes cell proliferation and migration in vitro. (a) The protein levels of FEN1 in Hep-3b and Hep-G2 after knockdown by si-FEN1 were detected by western blot. (b) Knockdown of FEN1 in Hep-3b and Hep-G2 significantly decreased cell proliferation compared with si-NC cells, performed by the CCK-8 assay. (c) Colony numbers of Hep-3b and Hep-G2 cells transfected with si-FEN1 were significantly lower than those transfected with si-NC, measured using the colony formation assay. (d) Suppression of FEN1 attenuated the proliferation of Hep-3b and Hep-G2 cells, measured by the EDU assay (magnification, ×100). Scale bar, 100 μm. (e) FEN1 suppression impaired HCC cell migration, as measured by the wound healing assay. , , and . Error bars indicate . (f) Protein levels of FEN1, c-Myc, survivin, and cyclin D1 after knockdown by si-FEN1. FEN1: flap endonuclease 1; HCC: hepatocellular carcinoma.

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