Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines

Figure 5

Immunohistochemical expression of selected proteins (more highly expressed in NTERA-2 than in TCam-2) in a tumor-free testis, GCNIS, seminoma, and embryonal carcinoma: A tumor-free testis and GCNIS show no marked differences in protein expression of PHF-6 and ENSA on immunohistochemical analysis (a, b, f, g). The expression of PHF-6 and ENSA in embryonal carcinomas (d, i) is markedly higher than that in seminomas (c, h; white arrow tumor cells, black arrow lymphocytes). The differences between seminomas and embryonal carcinomas in staining intensity (IRS) of PHF-6 € and ENSA (j) are significant.