Reduced Histone H3 Acetylation in CD4⁺ T Lymphocytes: Potential Mechanism of Latent Autoimmune Diabetes in Adults

Figure 1

The study scheme. A total of 56 subjects (28 LADA patients; 28 healthy control subjects) participated in this study. Peripheral blood mononuclear cells (PBMC) were isolated from each subject with the Ficoll. CD4⁺ T lymphocytes were isolated by positive selection using magnetic beads. Then, Global H3 and H4 acetylation was detected in CD4⁺ T lymphocytes. The mRNA level of histone acetyltransferases gene (P300 and CREBBP) and histone demethylases gene (HDAC1, HDAC2, and HDAC7) was detected by RT-PCR.