Mig-6 Gene Knockout Induces Neointimal Hyperplasia in the Vascular Smooth Muscle Cell

Figure 3

EGFR phosphorylation of vascular SMCs with regard to Mig-6 knockout. (a) Western blotting for phospho-EGFR in cultured vascular SMCs of Mig-6 ^d/d and Mig-6 ^f/f mice. pEGFR, phospho-EGFR; αSMA, α-smooth muscle actin. (b) Western blotting for EGFR and downstream signals according to transfection of siRNA into human vascular SMCs. siNS, transfection of nonspecific siRNA; siMIG-6, transfection of siRNAs against MIG-6; pEGFR, phospho-EGFR; tEGFR, total EGFR; pERK, phospho-ERK; tERK, total ERK; pAAK, phospho-Akt; tAKT, total AKT.

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