Effects of Different Intervention Factors on Vascular Endothelial Growth Factor-Induced Human Airway Smooth Muscle Cell Migration

Figure 4

Transwell assays detected the effect of VEGF on ASMC migration. (1) Transwell assays detected VEGF 20 ng·ml⁻¹ in the control, VEGF 40 ng·ml⁻¹, and PDGF 10 ng·ml⁻¹ groups. The effect of group on ASMC migration; PDGF 10 ng·ml⁻¹ was used as the positive control group. (a) A representative picture. (b) The histogram shows the number of migrating cells in each group, and the data are expressed as the mean ± standard deviation (M ± SD). (2) Transwell assays were used to detect the effect of VEGF (40 ng·ml⁻¹) and the control group on ASMC migration at 12 and 24 h. (c) A representative picture. The histogram in (d) shows the number of cells that migrated in the control and VEGF 40 ng·ml⁻¹ groups at 12 and 24 h, and the data are expressed as the mean ± standard deviation (M ± SD). NS indicates that the difference between groups is nonsignificant (), , and the difference between groups was significant ().

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