Case Report

Severe Atelectasis due to Aspirated Valproic Acid Tablet

Figure 3

mRNA expression of MUC2, MUC5AC, and MUC5B analyzed using real-time quantitative PCR. NCI-H292 cells were incubated with either the medium alone (control) or with the medium supplemented with valproic acid (5 mM; Cayman Chemical Company #13033) for 24 hours; mRNA was extracted thereafter. Relative gene expression was calculated using the ΔΔCt method with normalization to GAPDH. Data are representative of three independent experiments. Results represent the . Student’s -test was used to compare the groups.