Hemangioblastoma in the Lung: Metastatic or Primary Lesions?
Table 1
Results of immunohistochemistry studies on pulmonary hemangioblastoma.
Antibodies
Immunohistochemistry results
Clone/dilution/company
Inhibin
Stromal cell positive
R1/prediluted/VMSc
NSEa
Stromal cell positive
Polyclonal/1 : 100/Zymed
S-100
Stromal cell positive
Polyclonal/1 : 900/DACO
Vimentin
Stromal cell positive
V9/prediluted/VMSc
Calretinin
Rare stromal cell positive
Polyclonal/1 : 25/Introgen
Pancytokeratin
Negative
AE1/3/PCK26/prediluted/VMSc
EMAb
Negative
E29/prediluted/Cell Marque
CD10
Negative
SP67/prediluted/VMSc
Melan A
Negative
MART-1/prediluted/VMSc
Synaptophysin
Negative
SP11/prediluted/VMSc
Chromogranin
Negative
Polyclonal/1 : 1600/DACO
GFAP
Negative
Polyclonal/1 : 1600/DACO
CD68
Negative
KP1/1 : 3600/DACO
Lysozyme
Negative
Polyclonal/1 : 3600/DACO
CD1a
Negative
EP3622/prediluted/VMSc
CD34
Endothelial cell positive
QBend10/prediluted/VMSc
Ki-67
Estimated 5% of interstitial tumor cells positive
MIB1/1 : 25/DACO
NSE: neuron specific enolase
bEMA: embryonic membrane antigen
cVMS: Ventana Medical System Inc. Note: CD34, lysozyme, calretinin, and NSE are done on small tumor nodule; the rest are done on larger tumor nodule.