Research Article
A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study
Table 3
Robustness study data obtained from UPLC analysis.
| Robustness conditions | RT (min) | ASSAY (%) | System suitability data | Type | Rate | Theoretical plate | Asymmetry |
| Flow rate | 0.28 mL/min. | 1.25 | 99.85 | 2792 | 1.06 | 0.32 mL/min. | 1.06 | 101.99 | 2460 | 0.71 | Temperature | 28 | 1.05 | 98.95 | 2361 | 0.97 | 32 | 1.24 | 99.55 | 3194 | 1.67 | Mobile phase | 53 : 47 | 1.26 | 98.60 | 2984 | 1.05 | 57 : 43 | 1.11 | 99.51 | 2789 | 1.10 |
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