Isolation of Low Abundance Proteins and Cells Using Buoyant Glass Microbubble Chromatography
Figure 3
Nonspecific serum protein binding to Dynabeads, Sepharose and glass bubbles. Slurries with equivalent neurofilament heavy chain binding capacities were added to serum and processed as described in the Materials and Methods. Panel A shows the total protein eluted from the resins and glass bubbles and panel B shows the protein profile of eluted proteins by SDS-PAGE. Lanes 1, 2 and 3 are proteins eluted from protein A Dynabeads, Sepharose and glass bubbles, respectively. Each lane was loaded with 100 ng of eluted protein.
