Isolation of Low Abundance Proteins and Cells Using Buoyant Glass Microbubble Chromatography

Figure 2

Normalization of Protein A Resin and Glass Bubble Binding Capacities. Increasing concentrations of Cy5 labeled neurofilament heavy chain was added to 1 ml aliquots of low-IgG FBS as described in Materials and Methods. Slurries of protein A Sepharose (20 μL), Dynabeads protein A (10 μL) or glass bubbles conjugated with protein A (100 μL) and saturated with anti neurofilament antibodies were added to the tubes and processed as described in Materials and Methods. Closed squares (■) show the protein A glass bubbles, closed diamonds (♦) show the protein A Sepharose and closed triangles (▲) show the protein A Dynabeads.