Investigating Mutations to Reduce Huntingtin Aggregation by Increasing Htt-N-Terminal Stability and Weakening Interactions with PolyQ Domain

Table 4

Aggregation potentials of top 20 results for Htt-N-term 3-point mutations computed by PASTA.

Mutant	Best energy	%  -helix	% coil
Native		76.47	23.53
M1		70.59	29.41
M2		76.47	23.53
M3		76.47	23.53
M4		70.59	29.41
M5		76.47	23.53
M6		70.59	29.41
M7		82.35	17.65
M8		76.47	23.53
M9		76.47	23.53
M10		76.47	23.53
M11		64.71	35.29
M12		76.47	23.53
M13		76.47	23.53
M14		64.71	35.29
M15		70.59	29.41
M16		70.59	29.41
M17		76.47	23.53
M18		76.47	23.53
M19		76.47	23.53
M20		76.47	23.53

Mutant M7 results in the lowest energy, highest %  -helix, and lowest % coil.