Investigating Mutations to Reduce Huntingtin Aggregation by Increasing Htt-N-Terminal Stability and Weakening Interactions with PolyQ Domain

Table 3

Aggregation potentials of top 20 results for Htt-N-term 3-point mutations computed by TANGO.

Mutant	Amylo	Turn	Helix	Beta
Native	0.55	3.17	28.74	15.36
M1	4.18	6.31	1.26	20.41
M2	0.57	6.38	0.00	18.00
M3	0.57	6.16	0.00	20.56
M4	4.21	3.67	1.26	15.58
M5	0.57	3.75	0.00	13.17
M6	0.00	6.11	16.82	19.46
M7	0.57	3.53	0.00	15.73
M8	0.57	3.64	0.00	11.63
M9	0.00	5.87	9.52	26.75
M10	0.00	5.95	8.95	21.72
M11	0.56	5.92	7.97	19.19
M12	4.16	5.65	6.32	26.40
M13	0.56	5.75	1.85	21.45
M14	0.56	5.80	7.97	19.42
M15	0.08	6.54	0.00	20.13
M16	0.00	3.49	17.05	14.66
M17	4.16	5.53	6.32	26.63
M18	0.95	6.24	0.00	27.17
M19	0.57	5.63	1.85	21.68
M20	0.34	6.25	1.06	23.01

The Amylo column of the results returned by TANGO suggests that mutant M16 has the least amyloidogenicity.