Advances and Computational Tools towards Predictable Design in Biological Engineering

Box 2

Outstanding questions.

(i) What is still needed to fully understand the context-dependent variability of

   biological parts?

(ii) How can we develop accurate computational tools for the bottom-up biological

  engineering?

(iii) How can we support the construction of novel synthetic biology-based

  solutions?

(iv) How can we understand, model and predict cell-to-cell variability in the

  functioning of biological systems?

(v) If the fully predictable engineering of biological systems is not possible, how can

  we improve the efficiency of trial-and-error approaches?