Early Imaging Biomarker of Myocardial Glucose Adaptations in High-Fat-Diet-Induced Insulin Resistance Model by Using ¹⁸F-FDG PET and [U-¹³C]glucose Nuclear Magnetic Resonance Tracer

Figure 6

Western blotting and mRNA analysis of myocardium: AceCS1, Glut4, PARP, and LC3B protein expression and Slc2a4 and Acss2 gene expression in the perfusion hearts in HFD and control rats in 18 weeks and 36 weeks after high-fat diet feeding. AceCS1 protein expression and Acss2 gene expression did not show a significant difference between control and HFD in early and late stages. However, mRNA levels change in Glut4, albeit different in trend. Nonetheless, the protein expression of Glut4 increases in accordance with the ¹⁸F-FDG PET/CT. Decreased cleaved/prototype PARP and increased LC3B-autophagy activation in the myocardium of the HFD rats are noted.

(a)

(b)