Nuclear Imaging Study of the Pharmacodynamic Effects of Debio 1143, an Antagonist of Multiple Inhibitor of Apoptosis Proteins (IAPs), in a Triple-Negative Breast Cancer Model

Figure 1

D1143 induces apoptosis of human breast adenocarcinoma cells. (a) Viable MDA-MB-231 cells (%) after treatment with increasing concentration of paclitaxel (left panel) or D1143 (right panel) for 72 h. Paclitaxel and D1143 are expressed as log[concentration] for IC50 determination. Results are presented as mean ± SEM; . (b) Annexin-V+/7-AAD- (left panel) and Annexin-V+/7-AAD+ (right panel) MDA-MB-231 cells (%) after treatment with D1143 (0.3 µM, 1 µM, and 3 µM) or staurosporine (3 µM) for 6 h. Results are presented as mean ± SEM; ; , , . (c) Active caspase-3 positive MDA-MB-231 cells (%) after treatment with D1143 (0.3 µM, 1 µM, and 3 µM) or staurosporine (3 µM) for 6 h. Results are presented as mean ± SEM; ; , . (d) Bound/Total ^99mTc-HYNIC-Annexin V MDA-MB-231 cells (%) after treatment with D1143 (0.3 µM, 1 µM, and 3 µM) or staurosporine (3 µM) for 6h. Results are presented as mean ± SEM; ; .

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