MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management

Figure 1

(a) Purification of ⁸⁹Zr-DFO-LEM2/15 in a PD-10 column; the antibody elutes between volume fractions 2.5 and 5.0 ml. Inset: ITLC image illustrating RQP of the ⁸⁹Zr-DFO-LEM2/15 peak. 1 corresponds to the radioimmunoconjugate and 2 to the free radionuclide. (b) Percentage of bound radioactivity to LEM2/15 after 7-day incubation of ⁸⁹Zr-DFO-LEM2/15 with human serum at 37°C () and 4°C () and human plasma at 37°C () and 4°C (); data expressed as mean ± SD. (). Representative HPLC chromatogram of ⁶⁸Ga-DOTA-AF7p-1 before (c) or after (d) to Sep-Pak Light C18 cartridge purification. ⁶⁸Ga-DOTA-AF7p-1 is detected at 8.7 min by UV absorbance at 280 nm () and radioactivity detector ().

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