Spectral Unmixing Imaging for Differentiating Brown Adipose Tissue Mass and Its Activation

Figure 2

Spectral unmixing with CRANAD-29 for in vivo imaging. (a) Raw images of CRANAD-29 before spectral unmixing. (b) Unmixed autofluorescence signal. (c) Unmixed NIRF signal from BAT mass. (d) Unmixed NIRF signal from blood flow. (e) Merged image of unmixed #2 and #3. Note: for clarity, unmixed #1 was not merged.

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