MicroPET/CT Imaging of AXL Downregulation by HSP90 Inhibition in Triple-Negative Breast Cancer

Figure 5

RT-PCR and western blot analysis of tumor lysates and immunohistochemical staining of tumor samples. (a) RT-PCR of MDA-MB-231 tumor lysates shows that tumors from 17-AAG-treated mice had significant lower expression of mRNAs for EMT markers (vimentin, Twis1, Snai1, and Snai2; < 0.05, < 0.01, < 0.001). (b) Left panel: western blot analysis of AXL and vimentin expression in the tumor lysates. Right panel: quantification of protein blots of AXL and vimentin expression in the tumor lysates ( < 0.001). (c) IHC staining of AXL and vimentin expression of the tumor specimens from mice treated with vehicle or 17-AAG. Representative images are shown (scale bar: 20 µm, < 0.001, < 0.0001).

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