Research Article

MicroPET/CT Imaging of AXL Downregulation by HSP90 Inhibition in Triple-Negative Breast Cancer

Figure 3

Effects of 17-AAG on AXL expression in and motility of MDA-MB-231 cells. (a) Top panel: protein lysates from MDA-MB-231 cells treated with 17-AAG at the indicated doses for 8 h were immunoblotted for AXL; NT: untreated cells. β-Actin was used as a loading control. Bottom panel: protein lysates from MDA-MB-231 cells treated with 1000 nM 17-AAG and harvested at the indicated time points were immunoblotted, again using anti-AXL and anti-actin antibodies. (b) Wound-healing migration assay of MDA-MB-231 cells treated with 17-AAG (5 µM). The healing of the wounds, a measure of cell migration, was imaged at times 0, 12, 24, and 36 h (left). Representative images are shown. Percentage of the closed wound area at 36 h after treatment compared with that at 0 h (right). Results are expressed as means ± SD from three independent experiments ( < 0.0001; Student’s t-test).
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