Prevalence of bla_CTX-M and bla_TEM Genes in Cefotaxime-Resistant Escherichia coli Recovered from Tertiary Care at Central Nepal: A Descriptive Cross-Sectional Study

Table 5

Diagnostic evaluation of the phenotypical method on comparison with detection of bla_CTX-M genes associated with ESBL in cephalosporin-resistant E. coli.

Test characteristics (molecular method)	Phenotypic method combined disk diffusion method
TP (PCR = 36)	25
FP (PCR negative)	23
TN (PCR = 37)	17
FN (PCR positive but phenotypic negative)	11
Sensitivity	69.44%, 95% CI (51.89% to 83.65%)
Specificity	42.50%, 95% CI (27.04% to 59.11%)
PPV	52.08%, 95% CI (43.54% to 60.51%)
NPV	60.71%, 95% CI (45.64% to 73.99%)
Accuracy	55.26%, 95% CI (43.41% to 66.69%)

TP = True Positive, FP = False Positive, TN = True Negative, FN = False Negative, PPV = positive predictive value, NPV = negative predictive value.