Research Article
Differential Infectivity of Human Neural Cell Lines by a Dengue Virus Serotype-3 Genotype-III with a Distinct Nonstructural Protein 2A (NS2A) Amino Acid Substitution Isolated from the Cerebrospinal Fluid of a Dengue Encephalitis Patient
Figure 5
Infectivity of DENV-3 in mosquito and neural cell lines. DENV-3 antigen against structural (E) and nonstructural antigens (NS1, NS2B, NS3, NS4A, NS4B, and NS5) is shown as determined by using immunofluorescence (IFA) staining at 48 hours postinfection at a multiplicity of infection (MOI) of 0.01. DENV-3 isolated from the cerebrospinal fluid (CSF) specimen (CSF-11098, indicated as CSF) and the serum specimen (HP-5528, indicated as S) was separately inoculated to the C6/36, SKNSH, T98G, and N2A cell lines. Each figure represents a random field at a 200x magnification.
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