Differential Infectivity of Human Neural Cell Lines by a Dengue Virus Serotype-3 Genotype-III with a Distinct Nonstructural Protein 2A (NS2A) Amino Acid Substitution Isolated from the Cerebrospinal Fluid of a Dengue Encephalitis Patient

Figure 2

DENV-3 genome levels at 0, 24, 48, 72, 96, and 120 h postinfection (P.I). Virus genome levels were quantified by using real-time qPCR after infection at a multiplicity of infection (MOI) = 0.01 ((a)–(d)) and MOI = 0.001 ((e)–(h)). DENV-3 isolated from the cerebrospinal fluid (CSF) specimen (CSF-11098) and serum specimen (HP-5528) was separately inoculated to C6/36, SKNSH, T98G, and N2A cell lines. Viral genome copies number present in infected cells was determined at different time points and compared between a CSF sample and a serum sample. P value was determined by the t-test. P value 0.05 was considered significant.

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