Phenotypical and Genotypical Comparison of Clostridium difficile Isolated from Clinical Samples: Homebrew DNA Fingerprinting versus Antibiotic Susceptibility Testing (AST) and Clostridial Toxin Genes

Table 1

Primer sequences used for amplification of tcdA, tcdB, cdtA, cdtB, and gluD genes.

Gene	Primer	Sequence: 5 ⟶ 3′	Product size (bp)	Ref.
tcdA	TA1; TA2	5′-ATG ATA AGG CAA CTT CAG TGG-3′; 5′-TAA GTT CCT CCT GCT CCA TCA A-3′	624	[21]
tcdB	TB1; TB2	5′-GAG CTG CTT CAA TTG GAG AGA-3′; 5′-GTA ACC TAC TTT CAT AAC ACC AG-3′	412	[21]
cdtA	TCA1; TCA2	5′-TGA ACC TGG AAA AGG TGA TG -3′; 5′-AGG ATT ATT TAC TGG ACC ATT TG -3′	375	[22]
cdtB	TCB1; TCB2	5′-CTT AAT GCA AGT AAA TAC TGA G -3′; 5′-AAC GGA TCT CTT GCT TCA GTC -3′	510	[22]
GDH	gluD1; gluD2	5′-TGT CAG GAA AAG ATG TAA ATG TCT TCG AG-3′; 5′-TTA GTA CCA TCC TCT TAA TTT CAT AGC TTC-3′	1278	[21]