Detection and Quantification of Total and Pathogenic Vibrio parahaemolyticus in Anadara subcrenata in the Zhoushan Archipelago

Table 2

Primers and probes for multiplexed polymerase chain reaction-based detection of total and pathogenic Vibrio parahaemolyticus (Ward and Bej [12]).

Target gene	Primer or probe	Sequence^a	Amplicon size (bp)
tlh	F-tlh	5′-AAA GCG GAT TAT GCA GAA GCA CTG-3′	450
	R-tlh	5′-GCT ACT TTC TAG CAT TTT CTC TGC-3′
	P-tl952	5′-TEXR-AAG AAC TTC ATG TTG ATG ACA CT-BHQ2-3′^b
tdh	F-tdh170DG	5′-GTA RAG GTC TCT GAC TTT TGG AC-3′	229
	R-tdh403	5′-CTA CAG AAT YAT AGG AAT GTT GAA G-3′
	P-tdh-341R	5′-CY5-ATT TTA CGA ACA CAG CAG AAT GA-Iowa Black-RQ-3′^c
trh	F-trh82	5′-CCA TCM ATA CCT TTT CCT TCT CC-3′	207
	R-trh287	5′-ACY GTC ATA TAG GCG CTT AAC-3′
	P-trh275	5′-TET-TAT TTG TYG TTA GAA ATA CAA CAA T-BHQ1-3′^d

^aY = C or T; M = A or C; R = G or A. ^bTexR, sulforhodamine 101 (Texas Red) fluorescent dye; BHQ2, Black Hole-2 quencher dye. ^cCy5, carbocyanine fluorescent dye; Iowa Black-RQ, Iowa Black quencher dye. ^dTET, tetrachloro-6-carboxyfluorescein fluorescent dye.