Molecular Detection of Bartonella spp. in China and St. Kitts

Figure 2

Alignment of oligonucleotides for Bartonella PCR based on gltA used in this study. Two partial amplicons of polymorphic regions of Bartonella spp. based on gltA are shown. The base of B. henselae (L38987.1) shown at the top of the figure was complete with the coding sequences from 630 to 678 and 702 to 768. Dots indicate identical nucleotide sequence to that of B. henselae. Through the polymorphic region of Bartonella spp., different Bartonella species could be determined by genomic sequencing in this study. The number of nucleotide mismatches between B. henselae and other Bartonella species in the 116 polymorphic coding sequences is also noted (mismatch in coding sequences from 630 to 678 and 702 to 768 with about 5 and 23 nucleotides). In this polymorphic region, none of Babesia species had identical sequences.