Research Article
Sequential Treatment with Activin and Hepatocyte Growth Factor Induces FOXM1 to Promote Colorectal Cancer Liver Metastasis
Figure 4
FOXM1 depletion attenuates the effects of sequential activin and HGF treatment on gene expression and CRC liver metastasis. (a-b) Western blot (left) and qPCR (right) analysis of CXCR4 and AFP expression in HCT116 cells with indicated treatments. vs. control. (c) WT or FOXM1-depleted HCT116 cells subjected to sequential treatment with activin and HGF were injected into the spleen of the nude mice. Metastatic lesions in the liver were counted. . (d) Immunohistochemical analysis shows that FOXM1 knockdown reduces the expression of AFP and CXCR and liver metastasis in nude mice.
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