Research Article

EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-κB Signaling Pathway in Rats

Figure 4

After establishing the UC model, EGCG and TLR4 inhibitor E5564 intervened. The colonic tissue was collected and the total protein was extracted. Western blot assay was used to detect the expressions of TLR4, MyD88, and NF-κB protein expressions (a). The 18 colonic tissues were collected, the total RNAs were extracted, and the first strand of DNA was synthesized by reverse transcription. QRT-PCR was used to detect the expressions of TLR4, MyD88, and NF-κB at mRNA level (b). The colon tissue was collected, fixed in 10% formaldehyde, embedded, and sliced into sections. Immunohistochemistry was used to detect the TLR4, MyD88, and NF-κB expressions (c). Compared with control group, . Compared with UC group, .
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