Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin

<div>Re-5, Re-6, and Re-9 infected and spread within MDCK-TMPRSS2 and MDCK-MSPL cells. MDCK-TMPRSS2, MDCK-MSPL, MDCK + TPCK, and MDCK cells were infected with Re-5, Re-6, and Re-9, respectively (MOI = 0.01); cells were fixed 60 h after infection. Cells were incubated with Re-5, Re-6, and Re-9 antibodies and TRITC-labeled secondary antibody. Cells were observed under a Zeiss fluorescent microscope.</div>

Biotechnology Research International

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Figure 4: Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin 

Figure 4 | Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin 