Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin

<div>Stable expression of TMPRSS2 and MSPL in cell lines. (a) RT-PCR of MDCK-TMPRSS2 and MDCK-MSPL cells. Lanes 1 and 3: beta-actin; Lane 2: TMPRSS2; Lane 4: MSPL. (b) Western blot of MDCK-TMPRSS2 and MDCK-MSPL cells. Cell lysates were separated on SDS-PAGE and transferred to nitrocellulose membranes; TMPRSS2-FLAG and MSPL-FLAG fusion proteins were detected with FLAG antibody. (c) Green fluorescence of MDCK-TMPRSS2 and MDCK-MSPL cells was detected by flow cytometry. Both MDCK-TMPRSS2 and MDCK-MSPL cells were positive for green fluorescence.</div>

Biotechnology Research International

fig2

Figure 2

Figure 2: Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin 

Figure 2 | Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin 