Purification, Characterization of L-Methioninase from Candida tropicalis, and Its Application as an Anticancer

Table 1

Purifications steps, purification folds, and recovery yields of L-methioninase.

Purification step	Total protein (mg) ± SD	Total activity (units) ± SD	Specific activity (U/mg protein) ± SD	Enzyme recovery; (%)	Purification fold
Crude enzyme	3469.2 ± 89	5243.18 ± 78	1.5 ± 0.2	100	1
Heat treatment at 60°C for 10 min	2360.9 ± 94	4876.5 ± 70	2.06 ± 0.1	93.0	1.37
DEAE cellulose fractions (3–11)	87.0 ± 21	3219.6 ± 45	37.1 ± 11	61.4	24.73
Sephadex G-100 fractions (14–20)	22.65 ± 10	1467.41 ± 38	64.78 ± 7	27.98	43.19