Recovery of Bacillus licheniformis Alkaline Protease from Supernatant of Fermented Wastewater Sludge Using Ultrafiltration and Its Characterization

Table 4

Effect of enzyme inhibitor and chelator on protease activity.

Inhibitor/chelator (5 mM)	Relative enzyme activity (%)
Control	100
β-Mercaptoethanol	78 ± 3.8
p-chloromercuric benzoate (p-CMB)	91 ± 4.3
Phenylmethyl sulphonyl fluoride (PMSF)	0
Diisopropyl fluorophosphate (DFP)	10 ± 0.5
Iodoacetate	96 ± 4.4
Ethelene diamine tetra acetic acid (EDTA)	110 ± 5.6
Dithiotheritol	100 ± 4.8