Biochemistry Research International

Research Article

Cardiomyopathy-Related Mutations in Cardiac Troponin C, L29Q and G159D, Have Divergent Effects on Rat Cardiac Myofiber Contractile Dynamics

Figure 5

Comparison of myofilament C a 2 + sensitivity ( 𝑝 𝐶 𝑎 5 0 ) and cooperativity ( 𝑛 H ) of pCa-tension relationships in detergent-skinned fibers reconstituted with cTnCWT, c c T n C L 2 9 Q , or c T n C G 1 5 9 D . (a) Effects of RcTnC mutants on 𝑝 𝐶 𝑎 5 0 (b) Effects of RcTnC mutants on 𝑛 H . The Hill equation was fitted to the normalized pCa-tension relationships to estimate 𝑝 𝐶 𝑎 5 0 and 𝑛 H . One-way ANOVA was used to compare 𝑝 𝐶 𝑎 5 0 and 𝑛 H estimates in c T n C L 2 9 Q and c T n C G 1 5 9 D fibers with the data from cTnCWT fibers as controls. Values are expressed as mean ± SEM. Number of fibers tested in each group is as follows: c T n C W T , 𝑛 = 1 5 ; c T n C L 2 9 Q , 𝑛 = 1 0 ; c T n C G 1 5 9 D , 𝑛 = 1 4 . Minimal statistical significance was set at 𝛼 = 0 . 0 5 . *** 𝑃 < 0 . 0 0 1 .
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824068.fig.005b
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