Overexpression of PGC-1α Increases Fatty Acid Oxidative Capacity of Human Skeletal Muscle Cells
Figure 5
Effects of GW501516-treatment in human myotubes overexpressing PGC-1α on palmitic acid oxidation (a) and mRNA levels of genes involved in lipid and glucose metabolism (b). Myotubes infected with either empty vector or PGC-1α were treated with 10 nM GW501516 or control (0.1% DMSO) for 48 h. (a): The cells were incubated with 1 mL/well of [3H]palmitic acid and assayed for labeled acid soluble metabolites (ASM), as described in Section 2. Values are presented as means ± SEM ( experiments, each representing one donor, with 3 replicates each). (b): mRNA expression was assessed by RT-PCR as described in Section 2. Values are presented as means ± SEM ( experiments, each representing one donor, with 3 replicates each), normalized to the levels of housekeeping gene 36B4 in control cells infected with empty vector and treated with DMSO. A fold change ≥2 or ≤0.5 was considered an increase or decrease in expression level, respectively.
