TY - JOUR
A2 - Mostarica-Stojković, Marija
AU - Yuan, Fangchao
AU - Xu, Xuesong
AU - Wu, Yakun
AU - Duan, Shigang
AU - Wu, Hao
PY - 2019
DA - 2019/11/20
TI - Gastrodin Ameliorates Acute Rejection via IRE1α/TRAF2/NF-κB in Rats Receiving Liver Allografts
SP - 9276831
VL - 2019
AB - Background. Liver transplantation (LT) is currently an effective treatment for end-stage liver disease, but the occurrence of acute rejection (AR) is still the main problem to be solved. The present study aimed to evaluate the effect of gastrodin (GAS) on LT. Methods. Rat transplant models were established and divided into SHAM, LT, GAS-L (50 mg/kg GAS), and GAS-H (100 mg/kg GAS) groups. The liver function, inflammatory factors, liver histopathology, survival of rats, number of M2-type macrophages, liver cell apoptosis, and pathway proteins were assayed at 7 days and 14 days after the operations. Results. With increasing GAS concentrations, liver function, expression of proinflammatory factors in the liver, and expression of M2-type molecules in macrophages were significantly improved, and the survival time of rats was significantly prolonged (P<0.05). All rats treated with low or high doses of GAS were judged to have nondeterministic acute rejection. Flow cytometry showed that liver cell apoptosis was decreased significantly in the GAS-L and GAS-H groups after GAS administration compared with apoptosis and differentiation in the LT group (P<0.05). Expression levels of Caspase-3, Bad, and Bax proteins were decreased, and the expression of the antiapoptotic protein Bcl-2 was increased in the GAS-L and GAS-H groups (P<0.05). Mechanistically, the ERS-related IRE1α/TRAF2/NF-κB pathway was suppressed by GAS, and GAS acted mainly on intrahepatic macrophages to affect AR and reduce ROS production (P<0.05). Conclusion. GAS ameliorated AR by inhibiting the IRE1α/TRAF2/NF-κB pathway in LT.
SN - 2314-6133
UR - https://doi.org/10.1155/2019/9276831
DO - 10.1155/2019/9276831
JF - BioMed Research International
PB - Hindawi
KW -
ER -