Suppression of IL-6 Gene by shRNA Augments Gemcitabine Chemosensitization in Pancreatic Adenocarcinoma Cells

Figure 3

Effect of shIL-6 transfection and gemcitabine treatment on apoptosis of PANC-1 cells. Apoptosis assay was performed in the PANC-1 cells by flow cytometry analysis of Annexin V/PI staining 48 h after shIL-6 transfection followed by treatment with or without gemcitabine. ((a)–(d)) Representative results of flow cytometry analysis. (e) An average of 3 separate experiments, .

(a) NC

(b) shIL-6

(c) Gemcitabine

(d) shIL-6 + Gem

(e)