Research Article

Identification and Characterization of a Splicing Variant in the 5′ UTR of the Human TLR5 Gene

Table 3

Relative quantities of TLR5f to TLR5sh isoform in various human cell lines.

Cell lines
THP-1E6.1U937RamosA549HaCaTT98GMSCH460HEK293Caco-2

0.500.480.310.250.460.170.230.390.420.290.41
1.2
short]0.60.510.320.260.560.200.280.470.500.350.49

0.490.480.290.230.640.800.500.670.610.440.89
1.07
long]0.540.560.340.270.700.870.550.720.670.480.97

Sum1.141.070.660.531.261.070.831.191.170.831.46

Ratio
Long/short0.940.950.920.981.254.311.941.571.431.381.98

and are the proportions of each transcript over exon VI, calculated by the formula. short] and long] are the “real” expression proportions. and are the calculated correction factors. The ratios presented here, being expressed over exon IV+, show the ratio of short or long alternative variants. The expression level of short TLR5 transcripts was arbitrarily set to 1.