Research Article

Identification and Characterization of a Splicing Variant in the 5′ UTR of the Human TLR5 Gene

Figure 5

The relative expression of long/short TLR5 transcripts of THP-1 cells upon stimulation with IFN-γ. Total RNA was extracted from cells and cDNA was obtained from 2.5 μg total RNA. Quantitative real-time PCR was performed and ΔCt calculation with an internal reference (“never-spliced” exon of TLR5 gene) was used to normalize data. (a) Cells were stimulated with 10 ng/ml IFN-γ for various time points. (b) Cells were stimulated for 6 hours with various concentrations of IFN-γ. Bar graphs show relative gene expression ± SD. , , .
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