Identification and Characterization of a Splicing Variant in the 5′ UTR of the Human TLR5 Gene

Figure 4

Ratios of long TLR5 transcripts to short TLR5 transcripts in several different cell lines. Expression of long TLR5 transcripts was quantified by RT-qPCR using primers recognizing exon V (primer pairs were represented by solid arrows), while expression of short TLR5 transcripts was quantified using primers recognizing the boundary between exons IV and VI (primer pairs were represented by dashed arrows). Exon VII was used as a reference gene (primer pairs were represented by dotted arrows). The expression level of short TLR5 transcripts was arbitrarily set to 1. indicates as compared with THP-1.