Mining of Microbial Genomes for the Novel Sources of Nitrilases

Table 5

Comparison of physiochemical properties of aliphatic, aromatic, and predicted nitrilase from the average consensus values reported by Sharma and Bhalla [16].

Parameters	Average value for aliphatic	Average value for aromatic	1	2	3	4	5	6	7	8	9
Number of amino acids	352.2	309.8	338.0	331.0	326.0	260.0	280.0	310.0	315.0	342.0	319.0
Molecular weight (Da)	38274.0	33693.5	36154.9	36491.2	36364.7	27903.3	31464.1	34938.1	33821.5	37472.7	34678.7
Theoretical; pI	5.5	5.5	5.0	4.9	6.2	5.2	5.6	5.4	4.8	5.4	5.8
NCR	41.7	35.8	41.0	44.0	40.0	32.0	36.0	43.0	43.0	41.0	39.0
PCR	30.3	29.2	26.0	25.0	37.0	21.0	27.0	34.0	29.0	30.0	32.0
Extinction coefficients; (M^−1 cm^−1) at 280 nm	50213.3	43975.0	45295.0	33015.0	43890.0	35200.0	62465.0	53400.0	47900.0	38305.0	31775.0
Instability index	41.2	38.5	30.1	52.5	27.0	27.7	28.6	39.6	46.6	36.6	38.5
Aliphatic index	89.40	89.90	94.1	87.9	93.6	81.1	76.0	90.9	86.2	92.8	89.3
Grand average of hydropathicity (GRAVY)	00.10	00.01	0.027	−0.17	−0.14	−0.051	−0.283	−0.109	0.045	−0.052	−0.002

NCR: negatively charged residues; PCR: positively charged residues.