Research Article
Mining of Microbial Genomes for the Novel Sources of Nitrilases
Table 3
Manually designed motifs (MDMs) for aliphatic and aromatic nitrilases showing the presence of essential catalytic triad (E, K, and C).
| Nitrilases | Manually designed motif |
| Aliphatic | [FL]-[ILV]-[AV]-F-P-E-[VT]-[FW]-[IL]-P-[GY]-Y-P-[WY] | R-R-K-[LI]-[KRI]-[PA]-T-[HY]-[VAH]-E-R | C-W-E-H-[FLX]-[NQ]-[PT]-L | [VA]-A-X-[AV]-Q-[AI]-X-P-[VA]-X-[LF]-[SD] |
| Aromatic | [ALV]-[LV]-[FLM]-P-E-[AS]-[FLV]-[LV]-[AGP]-[AG]-Y-P | [AGN]-[KR]-H-R-K-L-[MK]-P-T-[AGN]-X-E-R | C-W-E-N-[HY]-M-P-[LM]-[AL]-R-X-X-[ML]-Y | A-X-E-G-R-C-[FW]-V-[LIV] |
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