Mining of Microbial Genomes for the Novel Sources of Nitrilases

Table 1

Composition of various media used to cultivate procured strains for nitrilase production.

Name of the organism	MTCC number	Composition ()	pH	Growth temperature
Shimwellia blattae; ATCC 29907	4155	Beef extract: 1.0 g; Yeast extract: 2.0 g; Peptone: 5.0 g; NaCl: 5.0 g; Agar: 15.0 g	7.0–7.5	37°C
Runella slithyformis; ATCC 29530	9504	Glucose: 1.0 g; Peptone: 1.0 g; Yeast extract: 1.0 g; Agar: 15.0 g; Glucose: 4.0 g	7.0–7.5	26°C
Geodermatophilus obscurus; DSM 43160	4040	Yeast extract: 4.0 g; Malt extract: 10.0 g; CaCO3: 2.0 g; Agar: 12.0 g	7.2–7.5	28°C
Nocardiopsis dassonvillei; DSM 43111	1411	Yeast extract: 4.0 g; Malt extract: 1.0; Glucose: 4.0 g; Agar: 20.0 g	7.2–7.4	28°C
Streptomyces albus; J1074	1138	Yeast extract: 4.0 g; Malt extract: 1.0 g; Glucose: 4.0 g; Agar: 20.0 g	7.2–7.4	25°C
Flavobacterium indicum; DSM 17447	6936	Tryptic soy broth with agar; (TSBA-100)	7.3–7.5	30°C
Saccharomonospora viridis; ATCC 15386	320	Yeast extract: 4.0 g; Malt extract: 1.0 g; Glucose: 4.0 g; Agar: 20.0 g	7.2–7.4	45°C
Sphingopyxis alaskensis; DSM 13593	7504	Beef extract: 1.0 g; Yeast extract: 2.0 g; Peptone: 5.0 g; NaCl: 5.0 g; Agar: 15.0 g	7.0–7.5	30°C
Gluconacetobacter diazotrophicus; ATCC 49037	1224	Yeast extract: 5.0 g; Peptone: 3.0 g; Mannitol: 25.0 g; Agar: 15.0 g	7.0–7.3	28°C
Escherichia coli; BL21 (DE3)	—	Yeast extract: 5.0 g; NaCl: 10.0 g; Casein enzymatic hydrolysate: 10.0 g	7.0–7.5	37°C

Negative control.