Research Article

Leaf Proteome Analysis Reveals Prospective Drought and Heat Stress Response Mechanisms in Soybean

Figure 5

A representative figure showing 2D-DIGE analysis of differentially expressed soybean leaf proteins in response to heat, drought, and drought plus heat stresses. For each sample set, 30 μg of sample protein was mixed with 1.0 μL of diluted Cy3 or Cy5 dye for labeling. A pooled protein sample containing equal amounts of all samples was labeled with Cy2. Labeled samples were subjected to isoelectric focusing (pH 4–7) followed by 12% SDS-polyacrylamide gels. Gel images were scanned using Typhoon TRIO (GE Healthcare, PA, USA) and were analyzed using Image QuantTL software (version 6.0, GE Healthcare, USA) and then subjected to in-gel analysis and cross-gel analysis using DeCyder software, version 6.5 (GE Healthcare). The spot numbering on the merged 2D-DIGE image indicates the protein spots that were found statistically significant between various treatments for mass spectrometric identification.