Improved Efficiency and Reliability of NGS Amplicon Sequencing Data Analysis for Genetic Diagnostic Procedures Using AGSA Software

Figure 5

A composite sample including 28 variants validated in Sanger was analysed both with AGSA software and with SeqNext, using the same threshold of 20%. AGSA detected all 28 variants and 10 false positive variants whereas SeqNext missed 1 real variant and reported 28 false positives.