Research Article

Improved Efficiency and Reliability of NGS Amplicon Sequencing Data Analysis for Genetic Diagnostic Procedures Using AGSA Software

Figure 2

Graphic representations of homopolymer flowgrams. Individual flowgrams of indel variants were generated by AGSA. The -axis represents the signal intensity computed during pyrosequencing. Red bars represent the number of reads for each intensity interval of 0.1; blue bars represent the percentage of variation as shown by AVA (intensity interval of 1). Distribution of standard flowgrams discriminates real indel (a) from artefacts (b).
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