RNA Sequencing Reveals Xyr1 as a Transcription Factor Regulating Gene Expression beyond Carbohydrate Metabolism

Figure 7

DNA binding of Xyr1 to the upstream regions of three genes upregulated after xyr1 deletion. Xyr1 functionally binds to the promoter regions from the mannosyl-glycoprotein endo-N-acetyl-β-D-glucosaminidase, the putative acid aspergillopepsin I, and the possible heat shock protein Hsp23-encoding genes endo T (a), pepA (b), and hsp23 (c), which were upregulated in Δxyr1 when cultured on the inducing medium. Strong gel shifts were observed after Xyr1 was added to the reactions. The protein-DNA complex increased with protein concentration. The amounts of purified Xyr1 (μM) used were as indicated and about 10 ng Cy5-labeled probe was added to each reaction. The specificity of shifts was verified by adding 100-fold excess unlabeled specific (S) and nonspecific (NS) competitor DNA. Purified GST (1 μM) was used as the negative control to exclude nonspecific binding by GST.

(a)

(b)

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