Research Article

Workflow for Genome-Wide Determination of Pre-mRNA Splicing Efficiency from Yeast RNA-seq Data

Figure 3

Splicing efficiency in the prp4-1 and prp40-1 mutants. Splicing efficiencies for all known introns (for 5′ and 3′ splice sites separately) were calculated using the pipeline described in Figure 1. (a, b) Results for the prp4-1 mutant and its corresponding wild-type strain [17]. (c, d) Results for the prp40-1 mutant and its corresponding wild-type strain [24]. Higher values correspond to more efficient splicing. Full circles represent values for introns with sufficient coverage (≥5 transreads and ≥5 reads covering intron end base); open circles represent low-confidence values for introns with low sequencing read coverage.
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