High Efficient Expression, Purification, and Functional Characterization of Native Human Epidermal Growth Factor in Escherichia coli

Figure 4

Tricine-SDS-PAGE analysis of purified recombinant hEGF prepared by self-cleavage treated with thiol DTT and one-step affinity chromatography. M: protein ladder marker shown in kDa on the left side of the panel; lane 1: supernatant solution after treatment in 20 mM DTT for 12 h; lane 2: the purified hEGF in flow through solution of the Ni-NTA column; lane 3: eluted solution with 500 mM imidazole.