Research Article
High Efficient Expression, Purification, and Functional Characterization of Native Human Epidermal Growth Factor in Escherichia coli
Figure 2
Expression of recombinant hEGF-SUMO-H10 and hEGF-Mxe-SUMO-H10. M: protein ladder marker shown in kDa on the left sides of panels, (a) SDS-PAGE analysis of the recombinant hEGF-SUMO-H10 produced in BL21(DE3), lane 1: whole cell sample after induction for 4 h, lane 2: supernatant sample after induction for 4 h; (b) SDS-PAGE analysis of the recombinant hEGF-Mxe-SUMO-H10 produced in BL21(DE3), lane 1: whole cell sample after induction for 4 h, lane 2: supernatant sample after induction for 4 h; (c) Western blot analysis of recombinant hEGF-Mxe-SUMO-H10 produced in BL21(DE3), lane 1: supernatant sample after induction for 4 h.
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